மருத்துவ நுண்ணுயிரியல் & நோய் கண்டறிதல்

Auramine rhodamine stain-a rapid and sensitive tool for detection of coccidian parasites

Abstract

Savitha Dharavath , Neelima , Padmaja , Umabala, Madhusudan Patil & Vijay Dharma Teja

Introduction: The increased number of documented human coccidian infections that are often indistinguishable from other forms of community-acquired diarrhoea, together with the possibility of treating some of them, suggests a need for proper diagnostic techniques to recover and identify the organism.

Materials and methods: 100 samples which were received in sterile wide mouth container were included in the study. The samples were subjected to macroscopic and microscopic examination by saline mount, iodine mount, modified acid fast stain and auramine and rhodamine staining.

Results: The prevalence of coccidian parasites in this study was observed to be 6%. Coccidian parasites reported were Cryptosporidia and Cystoisospora. Immunocompromised patients were observed to be susceptible to infection with coccidian parasites. Auramine rhodamine stain showed 100% agreement with modified acid fast stain.

Conclusion: This study concluded that Auramine stain is a better and more rapid stain than modified acid-fast stain for identifying coccidian parasites.