ஜர்னல் ஆஃப் இம்யூனோபயாலஜி

Cutaneous Wound Healing By Antibodies to Injured Tissue

Abstract

Jameson Frankel

The process of healing a wound involves an extremely well-ordered series of actions, including hemostasis, inflammatory cell infiltration, tissue regeneration, and remodelling. Following tissue destruction, wound healing occurs, thus we reasoned that antibodies might bind to injured tissues, facilitating the engulfment of damaged tissues by macrophages. Here, we demonstrate how B cells participate in wound healing by secreting antibodies against injured tissues. Transfer of spleen cells into splenectomized mice reversed the delay in wound healing caused by splenectomy. Additionally, splenectomized nude mice showed a delay in wound healing. Magnetic beads used to transfer enhanced B220+ cells sped up the healing process in mice with splenectomies. Magnetic beads used to transfer enhanced B220+ cells sped up the healing process in mice with splenectomies. Using anti-IgG1 that has been fluorescein isothiocyanate-labeled, we were able to identify immunoglobulin G1 (IgG1) binding to injured tissues 6–24 hours after the injury. The amount of IgG1 binding to injured tissues was decreased after splenectomy. Studies using immunoblotting showed multiple bands, which were diminished following splenectomy. We discovered that the strength of numerous bands was lower in the serum from splenectomized animals than in that from mice who underwent a sham operation using immunoprecipitation with anti-IgG linked to protein G.