Completed genome sequences are rapidly increasing for Rickettsia but most the genes in them does not have an assigned function. The present investigation mainly focused only on identifying the function of hypothetical proteins of Rickettsia massiliae MTU5 that were conserved in Rickettsia species. Nearly, 114 conserved hypothetical proteins were selected that were conserved in Rickettsia species. Function annotation was carried out by using databases like SMART, Interproscan, Pfam, JAFA, COG, and BLAST. Among 114 conserved hypothetical proteins only 35 proteins were annotated. The study motivated us to thoroughly study the genome of Rickettsia massiliae MTU5 by bioinformatics approach for categorizing the proteins whose function was unknown.
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