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LC-MS Analysis, Total Phenolics Content, Phytochemical Study and DPPH Antiradical Scavenging Activity of Two Cameroonian Propolis Samples

Abstract

Cecile Fabiola Balingui1*, Nyemb Jean Noel2, Talla Emmanuel2, Ngassoum Martin Benoit1, Happi Mouthe Gervais3 and AbdouramanBoubakary2

Two samples of Cameroonian propolis from Nyambaka (Adamawa) and Mveg (Nord-West) were collected for
some analysis. Six extracts were obtained by simple maceration with solvents of increasing polarity: hexane, ethyl
acetate (EtOAc) and the MeOH/H2O (80:20, v/v). Liquid Chromatography coupled with Mass Spectrometry (LCMS)
analysis revealed the presence of compounds such as: p-coumaric acid, cinnamyl ester, dimethylkuradine,
galangin, pinocembrin inside the both propolis. The total phenolics content (TPC) was evaluated using Folin-
Ciocalteu method on the EtOAc and MeOH/H2O (80:20, v/v) extracts of each sample. Nyambaka propolis has
the major results which ranged from (200.06 ± 0.17) mgGAE/100 g RM for EtOAc extract to 847.73 ± 0.15 mgGAE/100 g RM for MeOH/H2O (80:20, v/v). The antiradical activity of the different extracts was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical inhibition method and MeOH/H2O extract of Nyambaka propolis has the highest antiradical activity with an IC50 of 0.34 mg/mL.