உயர் இரத்த அழுத்த இதழ்: திறந்த அணுகல்

Mitochondrial Long Chain Fatty Acid Oxidation Related Enzyme Changes in Different Preeclampsia-Like Mouse Models

Abstract

Xiaoyan Ding, Zi Yang, Yiwei Han and Huan Yu

Background: Long-chain Free Fatty Acids (FFA) and long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) are related to some preeclampsia. We studied the relationship between LCHAD, carnitine palmitoyltransferase (CPT) I and II and FFA in different preeclampsia-like models.

Methods: Classic preeclampsia models with Nw-nitro-L-arginine-methyl ester (L-NA) or Lipopolysaccharide (LPS) injection, antiphospholipid syndrome model with β2 glycoprotein I (β2GPI) injection, and ApoC3 mice with abnormal fatty acid metabolism (ApoC3+NS and ApoC3+L-NA) were used. Controls were saline injected. At days 14 and 18 of pregnancy, serum FFA levels were compared and placental and hepatic LCHAD, CPT I and II mRNA and protein were detected using real-time quantitative PCR and western blot.

Results: Serum FFA levels increased significantly except in LPS, compared to controls (P<0.05). CPT I mRNA and protein increased significantly in the liver and placenta of L-NA and β2GPI and liver of ApoC3+L-NA (P<0.05). CPT II mRNA and protein increased significantly in the liver and placenta of L-NA and the liver of ApoC3+L-NA and β2GPI (P<0.05). LCHAD mRNA and protein increased significantly in the liver and placenta of ApoC3+NS, ApoC3+L-NA and β2GPI, and decreased significantly in L-NA (P<0.05). Serum FFA levels positively correlated with liver CPT I mRNA and protein in all groups (P<0.05) and negatively correlated with liver LCHAD mRNA and protein expression in all groups except in β2GPI group (P<0.05).

Conclusions: Changes and correlations in FFA levels and LCHAD, CPT I and II were different according to preeclampsia-like models. β-oxidation disorders may occur at different stages.