Badal Saha
Microbial metabolism is heavily influenced by environmental factors. While these can be well controlled in the laboratory, large-scale bioreactors are distinguished by inhomogeneities and, as a result, dynamic conditions for the organisms. The mechanistic response of Saccharomyces cerevisiae to frequent perturbations in industrial bioreactors is still unknown. To investigate the adaptations to prolonged dynamic conditions, we used previously published repeated substrate perturbation regime experimental data, supplemented it with proteomic measurements, and combined the two for modeling approaches. Data from multiple sources, including quantitative metabolize, enrichment, and flux quantification, were combined. Kinetic metabolic modeling was used to investigate the dynamics of the relevant intracellular metabolic response. A previously developed model of yeast central carbon metabolism was expanded, and various subsets of enzymatic kinetic constants were estimated.
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