Noah George
SEM images of both mineralizing (subclone 4) and nonmineralizing (subclone 24) MC3T3-E1 cells with very dense colonies after 28 days of induction medium exposure are seen in the given figure. In particular, on the flat substrates, the cells appeared more rounded than expected. This may be because, to avoid objects in the XRD results, only a mild fixative, ethanol, was used. The osteoblasts tended to have a larger μm micropatterned Si region of distribution, with various filopodia spreading from several of the cells. Biomineral clusters have also been found in cells, subclone 4, but not subclone 24, identical to previous studies, cells. More cells were shown to be detected on the 20 μm micropatterned Si tightly connected on the ridges than in the valleys.
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