மூலக்கூறு உயிரியல்: திறந்த அணுகல்

Association of Angiotensin Converting Enzyme (ACE) Gene Polymorphisms and Risk of Diabetic 2 Among Patients Visiting Bahirdar Felegehiwot Referral Hospital North West, Ethiopia

Abstract

Nega Berhane, Tadele Tamiru, Wagaw Sendeku and Desalegne Menegesha

Background: Type 2 diabetes (T2DM) affect large population. There are many genetic and non genetic factors associated for the occurrence of T2DM. Genes of the renin angiotensin system angiotensin converting enzyme insertion/deletion gene polymorphism has been associated with the risk of type 2 diabetes. The aim of the present study was to investigate the association of angiotensin converting enzyme gene polymorphism, risk of type 2 diabetes.
Methods: A total of 222 subjects (111 T2DM and 111 healthy controls) were collected from Bahir Dar Felegehiwot Referral Hospital and Bahir Dar town, respectively by using non-probability purposive sampling techniques. Minidray fully automated analyzer was used for biochemical tests. The ACE I/D genotypes were identified by Polymerase chain reaction (PCR) using appropriate primers and PCR reaction conditions.
Results: The present study revealed that the frequency of DD genotype and D allele were higher in type 2 diabetes mellitus compared to healthy controls (DD, 64.2% vs.35.6% P<0.001) and (D, 79.3% vs.59.9% P<0.001). DD genotype showed threefold increase risk towards T2DM as compared to II Genotype (OR: 2.984, CI: 1.332-6.689, P<0.02). The D allele carriers had five times high risk of getting diabetic as compared to I allele. (OR, D 2.178; CI: 1.168-3.232 P<0.001 vs.I OR, 0.459; CI: 0.309-0.681 P<0.001).
Conclusion: Нis study indicated that the frequency of ACE DD genotype and D allele were markedly higher in patients with type 2 diabetes