..

இம்யூனோ கெமிஸ்ட்ரி & இம்யூனோபாதாலஜி

ஐ.எஸ்.எஸ்.என்: 2469-9756

திறந்த அணுகல்
கையெழுத்துப் பிரதியை சமர்ப்பிக்கவும் arrow_forward arrow_forward ..

Improved Immuno-Detection of a Low-Abundance Cyclophilin Allows the Confirmation of its Expression in a Protozoan Parasite

Abstract

Patricia L Bustos, Alina E Perrone, Natalia A Milduberger and Jacqueline Bua

Protein samples can be challenging to analyze due to the presence of high-abundance proteins masking lowabundance proteins of interest, such as biomarkers and novel physiological mediators. Cyclophilins are chaperones involved in the cis/trans isomerization of peptidyl-prolyl bonds in peptides or proteins and have been found in every organism sequenced to date. Although considerable progress has been made in the characterization of some cyclophilins expressed in diverse parasites invading humans, the main aspects of low-abundance members of this family remain unknown. In the present work, we present that the combined strategy of using more specific antibodies and increasing the presence of subcellular proteins in the sample, allowed us to confirm the expression of a 21.1 kDa cyclophilin for the first time in Trypanosoma cruzi.

இந்தக் கட்டுரையைப் பகிரவும்

குறியிடப்பட்டது

arrow_upward arrow_upward