ஜர்னல் ஆஃப் மாலிகுலர் பயோமார்க்ஸ் & நோயறிதல்

Presence of Phosphorylated Tau Protein in the Skin of Alzheimer´s Disease Patients

Abstract

Rodríguez-Leyva Ildefonso, Chi-Ahumada Erika, Calderón–Garcidueñas Ana Laura, Medina-Mier Verónica, Santoyo Martha E, Martel-Gallegos Guadalupe, Zarazúa Sergio , Carrizales Juan and Jiménez-Capdeville María E

Background: The presence of misfolded proteins in the brain is the hallmark of neurodegenerative diseases. Protein aggregates could have systemic expression and might be found in several tissues including the skin. Objective: To demonstrate the presence of phosphorylated Tau (p-Tau) in the skin cells of patients with Alzheimer´s Disease (AD). Material and methods: Antibodies against p-Tau (PHF, phosphorylated at S296 and AT8, phosphorylated at S202) were assayed in biopsied tissue from the retro-auricular area in 49. subjects: 20 with AD, 12 with nondegenerative dementia and 17 age-matched controls. Light and confocal microscopies were employed to localize Tau protein by immunohistochemistry and their presence in the skin was confirmed through Western blots. Results: The skin biopsy taken from AD patients presented significantly higher levels of p-Tau (AT8: hyperphosphorylated at Ser 202) when compared both to control subjects and patients with non-degenerative dementia (p<0.001). Conclusion: This study demonstrates the presence of p-Tau in skin biopsies by immunoreactivity. This procedure could be used to support the clinical diagnosis of AD in living patients.