Binandeh M
The project, based on large studies in the context of the application nanoparticles in biological science and medicine. So, Firstly the magnetic nanoparticles (MNPs) Fe3O4 synthesized by a chemical Co-precipitation method with a cover layer of silica (to prevent excess oxidation and increases the sufficiency of the surface of the nanoparticles) that in the size of 20 nm were prepared. Then the core/shell structure of it detected by the analyses of the SEM, FT-IR. Purpose of this production was for using of the full performance level of nanoparticles in absorption of antibiotics, ampicillin (amp), thus that normal value is 100 μg.ml-1 of amp solved in 20 mg of MNPs on terms of room temperature and normal time 0.25 hr. The resulting sample was tested by the analysis UV-Vis spectrophotometer and the results obtained indicated that amp absorbed is stabilized 85% up on the surface of nanoparticles. The resulting link between those two, the kind of bond-electrostatic which was proved by the analysis of EDX. Eventually this combination to processing bactericidal was used. In this case, MNPs-amp combination in the normal value of 20 μl of a bacteria culture model was added during overnight (In-vitro). More, the results showed that 95% of the bacteria were killed. According to the results obtained, as antibacterial properties of MNPs-amp, became apparent. Lastly, MNPs was separated by magnet which controlled by external magnetic field. So it can be targeted to a system for the transfer between nanoparticles to bacteria for specification of quality antibacterial.
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